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If you're using photoshop, and you want to make your flower into a brush, put a Hue>Saturation adjustment layer over it. In the dialog, drag the Saturation slider all the way to the left to desaturate the picture (turn it to grey-scale).
If your background was white to begin with, then you can proceed to make your brush below. If your background was colored, you may have to add a Brightness/Contrast adjustment layer and adjust it until the background is white.
Merge the layers. Use your Rectangular Marquee tool and draw a rectangle around the flower. Choose Edit>Define Brush Preset to make the brush.
Tandika
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